The mTOR/PDK1/4EBP1/TEP1 pathway, including PDK1, p-PDK1, 4EBP1, Immunohistochemistry was used to detect the level of PDK1, p-PDK1, 4EBP1, TEP1, Ki67, and the proliferation index.

The induction of apoptosis by spautin-1 and its effect on the mTOR/PDK1/4EBP1/TEP1 pathway were evaluated by Western blotting. The proliferation, invasion, and apoptosis of cells were measured by colony formation assay, a cell counting kit-8, wound-healing assay, and flow cytometry, respectively. The expression of the proteins was evaluated by Western blotting. We investigated the role of the mTOR/PDK1/4EBP1/TEP1 signaling pathway in the growth of glioblastoma cells and explored the underlying molecular mechanisms. The molecular mechanisms of glioblastoma growth remain largely unclear.

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